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Journal: Infection and Immunity
Article Title: Mouse Peptidoglycan Recognition Protein PGLYRP-1 Plays a Role in the Host Innate Immune Response against Listeria monocytogenes Infection
doi: 10.1128/IAI.00466-10
Figure Lengend Snippet: Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Article Snippet:
Techniques: Concentration Assay, Transfection, Control, Plasmid Preparation, Infection, Bacteria, Labeling