mouse hepatocyte cell line nmuli cells Search Results


90
DS Pharma Biomedical mouse hepatocyte cell line nmuli cells
Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. <t>NMuLi</t> <t>hepatocyte</t> cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Mouse Hepatocyte Cell Line Nmuli Cells, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nissui Pharmaceutical mouse hepatocyte cell line nmuli cells
Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. <t>NMuLi</t> <t>hepatocyte</t> cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.
Mouse Hepatocyte Cell Line Nmuli Cells, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse hepatocyte cell line nmuli cells/product/Nissui Pharmaceutical
Average 90 stars, based on 1 article reviews
mouse hepatocyte cell line nmuli cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.

Journal: Infection and Immunity

Article Title: Mouse Peptidoglycan Recognition Protein PGLYRP-1 Plays a Role in the Host Innate Immune Response against Listeria monocytogenes Infection

doi: 10.1128/IAI.00466-10

Figure Lengend Snippet: Increased elimination of L. monocytogenes in PGLYRP-1-overexpressing hepatocytes. NMuLi hepatocyte cells seeded at a concentration of 2 × 105 cells/well were transfected with pEGFP-C2/PGLYRP-1 or pEGFP-C2 control vector. Both transfected cells were infected with L. monocytogenes for 30 min, and cell cultivation was continued with the elimination of extracellular bacteria by gentamicin. After 6 h of cultivation, L. monocytogenes cells were labeled with rhodamine, and rhodamine-labeled bacterial numbers in GFP-labeled cells were counted and the ratio of the bacterial number to the cell number was calculated. An asterisk indicates a significant difference from the pEGFP-C2-transfected group at P < 0.05.

Article Snippet: Mouse hepatocyte cell line NMuLi cells (DS Pharma Biomedical, Osaka, Japan) were maintained in DMEM with 10% (vol/vol) FCS.

Techniques: Concentration Assay, Transfection, Control, Plasmid Preparation, Infection, Bacteria, Labeling